Model Line for HPGE Systems
- 1000 A System (Standard System)
- 1000 S System (Spectral System)
Technical Data
Electrophoresis
- Power modes: Constant voltage, V/cm, current or power
- Voltage range: 0 - 1000 V (accuracy ±1.0%)
- Field strength in gel: 0 - 80 V/cm (±1.0%)
- Current: 0 - 50 mA (±1.0%)
- Power: 0 - 50 W (±1.0%)
- Cooling Capacity: 20 W (±1.0%)
- Temperature: 0 - 70°C (±0.5°C), in 0.1°C
steps
- Field Inversion: Programmable, 1-3600 sec switch time; 0 - 70
V/cm (V effective) Pre-electrophoresis: Programmable
- Sample application: Programmable
- Sample concentration: Programmable (optional)
- Zone retrieval: Programmable (optional)
- In-Gel assay: Programmable (optional)
- Detection
- Sensitivity (DNA ): < 1 ng (1 kb fragment, EtBR-stained)
- Sensitivity (Protein): < 5 ng (BSA monomer, FITC)
- Linear Dynamic range: 10^4
- Gel background substraction: < 10^2
- Spatial resolution: 100 µm
- Scan frequency: Programmable, max. 60 scans / run
- Excitation spectrum 240 - 380 nm lambda max = 302 nm, min.energy
at 254 nm and 280 nm
- Excitation selectable: No
- Emission range: 490 - 650 nm (HPGE 1000 A)
-
450 -700 nm (HPGE 1000 S)
- Emission selectable: No
(HPGE 1000 A)
- Yes,
480-680 nm in 5 nm steps (HPGE 1000S)
- EMS wavelength indicator: No (HPGE 1000 A)
- Yes,
EMS maximum (HPGE 1000 S)
Gel and Buffers
- Gel format: 8 independent lanes, 12 cm length, 3 mm and 1.5
mm thickness
- Gel volume: 100 mL
- Sample well volume: 25 µL
- Sample volume: 10 - 25 µL
- Concentrator volume: 1 - 20 mL (optional)
- Buffer volume: 500 mL each reservoir
Software
- Operating software: Microsoft® Windows 3.1x/95/98/NT4.0
-based; User-level controlled access to system functionality; LIMS
environment permits data validation.
- Data display: Zones (bands) & electropherogram graphs; single
scan & scan history; comparison of multiple gels; live display of entire
gel
- Analysis software: Identification, quantification and comparison
of zones; Calibration: Quantification, Molecular size analysis, Isoelectric
points, Biological activity (optional)
- GLP-compliance: Yes (ANSI/ASQC)
Hardware
- Dimensions: 31H x 51W x 40D cm (12 x 20 x16" )
- Weight: 30 kg (66 lb)
- Power requirement: 100-240Vac, 50/60 Hz
- Ambient conditions: 10 - 30°C, 80% maximum relative humidity
- Computer system: Pentium II Multimedia PC (32MB RAM, 4MB VRAM, 4.3/6.8GB Harddrive, 1.4MB 3.5" FloppyDrive, 12x/24x CD-ROM, Modem,
Sound); Display: ViewSonic 6E 14" CRT (HiColor,1024x768); Printer: Canon BJC4300/4500 Color Bubblejet (360/720 dpi)
The HPGE 1000 System from LabIntelligence is
a fully automated, computer-controlled real-time gel electrophoresis system
for the life sciences. LabIntelligence is located
in Belmont, California, U.S.A. For more information, call (+1) 650-654-9908
!
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