HPGE Reagents Specifications
-
HPGE 1000 System: Product Data
Sheets © LabIntelligence 1989-1999
- Last update: 03/01/1999
-
References and Standards
840-0300-00 Bovine Serum Albumin
Size Standard, FITC-labeled
- Product Specification Sheet
- Contains the following BSA oligomers (FITC-labeled):
- BSA Monomer 67400 BSA Dimer 134800 BSA Trimer 202200 BSA Tetramer 269600
BSA Pentamer 337000 (BSA Hexamer 404400) (BSA Heptamer 471800)
- The zones in parenthesis are not always visible at all gel concentrations
(invisible in more restrictive gels)
- Concentrations:
- Undiluted stock solution: 0.4 µg BSA monomer/µl (= 400
ng BSA/µl) All dilutions in 20 mM Tris, 20 mM Tricine, 0.1% SDS,
pH 8.60, conductivity 770 µS.
- Calibration Kit Vials:
- 1+24 (Dil. 25) 16 ng BSA/µl 1+49 (Dil: 50) 8 ng BSA/µl
1+99 (Dil:100) 4 ng BSA/µl
- Recommended load:
- 20-25µl /lane (best SDS concentration: 0.2% - 0.4% w/v)
- For use with the HPGE sytem
- Note: This oligomer preparation can be used in a single concentration
to calibrate for both, size AND quantification, since the Dimer is 1/2
of the Monomer concentration.
- Keep refrigerated at 4°C and protected from light!
- Specifications subject to change.
Concentrations may vary slightly lot dependent.
- Please refer to the attached
sheet in each package for actual concentrations!
840-0302-00 Lactalbumin
/ Bovine Serum Albumin Size Standard, dual-labeled
- Product Specification Sheet
- Contains the following LAl oligomers (Cascade Blue-labeled):
- LAl Monomer 14400 LAl Dimer 28800 (LAl Trimer 43200)
- Contains the following BSA oligomers (FITC-labeled):
- BSA Monomer 67400 BSA Dimer 134800 BSA Trimer 202200 BSA Tetramer 269600
BSA Pentamer 337000 (BSA Hexamer 404400) (BSA Heptamer 471800)
- The zones in parenthesis are not always visible at all gel concentrations
(invisible in more restrictive gels)
- Concentrations:
- Undiluted stock solution: 0.25 µg Lactalbumin monomer/µl
(= 250 ng LAl/µl) 0.4 µg BSA monomer/µl (= 400 ng BSA/µl).
All dilutions in 20 mM Tris, 20 mM Tricine, 0.1% SDS, pH 8.60, conductivity
770 µS.
- Calibration Kit Vials:
- 1+24 (Dil. 25) 10 ng LAl/µl, 16 ng BSA/µl 1+49 (Dil: 50)
5 ng LAl/µl, 8 ng BSA/µl 1+99 (Dil:100) 2.5 ng LAl/µl,
4 ng BSA/µl
- Recommended load:
- 20-25µl /lane (best SDS concentration: 0.2% - 0.4% w/v)
- For use with the HPGE system
- Note: This oligomer preparation can be used in a single concentration
to calibrate for both, size AND quantification, since the Dimer is 1/2
of the Monomer concentration. Dual-label standards can be used also for
multi-color calibration of the fluorescence spectrophotometer (Blue/green).
- Keep refrigerated at 4°C and protected from light!
- Specifications subject to change.
Concentrations may vary slightly lot dependent.
- Please refer to the attached
sheet in each package for actual concentrations!
The HPGE 1000 System from LabIntelligence is
a fully automated, computer-controlled real-time gel electrophoresis system
for the life sciences. LabIntelligence is located
in Belmont, California, U.S.A. For more information, call (+1) 650-654-9908
!
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Last
update: 03/01/1999 
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